Sliding clamp

A DNA clamp, also known as a sliding clamp , is a protein fold that serves as a processivity-promoting factor in DNA replication. As a critical component of the DNA polymerase III holoenzyme, the clamp protein binds DNA polymerase and prevents this enzyme from dissociating from the template DNA strand. This leads to remarkable increases in efficiency.

For instance, by tethering their DNA polymerase to a sliding clamp , bacteria increase . Sliding clamps allow DNA polymerases to remain attached to their DNA template. This lecture explains about the role of clamp loader and sliding clamp in dna replication.

Although these proteins have different oligomeric states, they all possess a general pseudo-sixfold ring-shaped structure. To achieve the high degree of processivity required for DNA replication, DNA polymerases associate with ring-shaped sliding clamps that encircle the template DNA and slide freely along it. The closed circular structure of sliding clamps necessitates an enzyme-catalyzed mechanism, which not only opens them for assembly . Looking for online definition of sliding clamp in the Medical Dictionary?

Meaning of sliding clamp medical term. What does sliding clamp mean? The elongation factor beta- clamp , also called . Structures: Beta sliding clamp (P0A988).

Escherichia coli (strain K12).

Crystal structure of a DNA polymerase sliding clamp from a Gram-positive bacterium. DNA sliding clamps are rings that tether certain enzymes to DNA. How clamp proteins slide on DNA has remained a mystery. A new crystal structure, together with molecular dynamics and NMR studies, has revealed how the human PCNA clamp slides on DNA.

Confers DNA tethering and processivity to DNA polymerases and other proteins. Acts as a clamp , forming a ring around DNA (a reaction catalyzed by the clamp – loading complex) which diffuses in an ATP-independent manner freely and bidirectionally along dsDNA. Initially characterized for its ability to contact the catalytic . DNA Replication in Humans: Loading and unloading the sliding clamp primase. Primase then leaves the template, and DNA polymerase takes over, extending the RNA primer with DNA nucleotides as it synthesizes the new DNA chain.

RNA primers are removed and replaced with DNA later in replication. However, its processivity becomes quite high when it is tethered to DNA by a sliding clamp. Because the eukaryotic sliding clamp was first detected as an antigen in proliferating cells before its function was known, it was called proliferating cell nuclear antigen (PCNA).

This name is still used but the more descriptive name of . The brace proteins recognize the -end of the primer in an R-loop or D-loop, and load the sliding clamp. This can be the initial primer at the origin of . Description: Used to facilitate quicker mould changes the Power Sliding Clamp offers an incredibly cost effective solution. The first sliding clamp structure determined was E. Chromosomal replication is an essential process in all life. The griR gene encodes an additional copy of the DNA polymerase III beta subunit (also called sliding clamp ), which shows amino acid identity to the housekeeping sliding clamp of S.

Bacterial DNA clamps observed to date form . The sliding clamp is known to tether DNA polymerase III to the DNA during DNA synthesis and thus provides processivity to . The ubiquitous sliding clamp facilitates processivity of the replicative polymerase and acts as a platform to recruit proteins involved in replication, recombination and repair. While the dynamics of the E. Clamp loaders load ring-shaped sliding clamps onto DNA where the clamps serve as processivity factors for DNA polymerases. In the first stage of clam.

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